Testing methods for melamine and cyanuric acid 三聚氰胺与三聚氰酸的监测方法

The Food Safety and Inspection Service (FSIS) of the United States Department of Agriculture (USDA) provides a test method for analyzing cyromazine and melamine in animal tissues in its Chemistry Laboratory Guidebook which "contains test methods used by FSIS Laboratories to support the Agency's inspection program, ensuring that meat, poultry, and egg products are safe, wholesome and accurately labeled
美国农业部食品安全检测局提供了一种检测动物器官中三聚氰胺和三聚氰酸的检测方法。在其化学实验室手册中指出“食品安全检测局提供方法确保在肉，家禽和蛋类食品的安全”
http://www.fsis.usda.gov/ophs/clg/Cyromazine.pdf
http://www.fsis.usda.gov/science/Chemistry_Lab_Guidebook/index.asp


FDA公布的用高效液相色谱测定三聚氰胺的方法
HPLC Determination of Melamine, Ammeline, Ammelide, and Cyanuric Acid Contamination in Wheat Gluten and Rice Protein Concentrate

Robert Smith, Jimmie Weigand, and Joan Nandrea. US FDA Laboratory, Lenexa, KS

Wheat gluten or rice protein concentrate was extracted with pH 2.5, 50 mM phosphate buffer by stirring vigorously for four hours at 60°C. The extract was injected onto a combination of two columns in series (a short strong cation exchange column and a C18 column) and eluted using a 50 mM phosphate pH 2.5 buffer. The limit of detection (LOD) and limit of quantification (LOQ) were estimated from the noise level to be approximately 100 µg/mL and 400µg/mL respectively for melamine, ammeline, and cyanuric acid in the original material. Ammelide LOD and LOQ were estimated at 10µg/mL and 40µg/mL respectively. Spike recoveries were 98% to110%. This method is useful for quantitation in the range of 0.1% to 5% for melamine and cyanuric acid, and from 400 ppm to 10,000 ppm for ammeline and from 50 ppm to 10,000 ppm for ammelide in the wheat gluten or rice protein.

FDA公布的用气相和质谱联用测三聚氰胺
http://www.fda.gov/cvm/GCMSMelamine.htm